Colorectal cancer (CRC) is one of the leading causes of death by cancer worldwide. Bowel cancer screening programs enable\nus to detect early lesions and improve the prognosis of patients with CRC. However, they also generate a significant number\nof problematic polyps, e.g., adenomas with epithelial misplacement (pseudoinvasion) which can mimic early adenocarcinoma.\nTherefore, biomarkers that would enable us to distinguish between adenoma with epithelial misplacement (pseudoinvasion) and\nadenoma with early adenocarcinomas (true invasion) are needed. We hypothesized that the former are genetically similar to\nadenoma and the latter to adenocarcinoma and we used bioinformatics approach to search for candidate genes that might be\npotentially used to distinguish between the two lesions. We used publicly available data from Gene Expression Omnibus database\nand we analyzed gene expression profiles of 252 samples of normal mucosa, colorectal adenoma, and carcinoma. In total, we\nanalyzed 122 colorectal adenomas, 59 colorectal carcinomas, and 62 normal mucosa samples. We have identified 16 genes with\ndifferential expression in carcinoma compared to adenoma: COL12A1, COL1A2, COL3A1, DCN, PLAU, SPARC, SPON2, SPP1,\nSULF1, FADS1, G0S2, EPHA4, KIAA1324, L1TD1, PCKS1, and C11orf96. In conclusion, our in silico analysis revealed 16 candidate\ngenes with different expression patterns in adenoma compared to carcinoma, which might be used to discriminate between these\ntwo lesions.
Loading....